Publication

We have developed novel technologies such as microfluidic islet chips and imaging chemical probes/bio-sensors to investigate the regulation of islet Ca$^{2+}$ activity patterns and hormone secretion by intra-islet paracrine interactions. These studies also involved the development of quantitative data analysis methods and mathematical modeling.

Highlighted

Pancreatic α and β cells are globally phase-locked

Huixia Ren, Yanjun Li, Chengsheng Han, Yi Yu, Bowen Shi, …, Shufang Wu, Xiaojing Yang, Sneppen Kim, Liangyi Chen, Chao Tang

Nature Communications  ·  28 Jun 2022  ·  doi:10.1038/s41467-022-31373-6

Readily releasable β cells with tight Ca2+–exocytosis coupling dictate biphasic glucose-stimulated insulin secretion

Xiaohong Peng, Huixia Ren, Lu Yang, Shiyan Tong, Renjie Zhou, …, Tao Xu, Chao Tang, Zhixing Chen, Huisheng Liu, Liangyi Chen

Nature Metabolism  ·  26 Jan 2024  ·  doi:10.1038/s42255-023-00962-0

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2024

PDX1+ cell budding morphogenesis in a stem cell-derived islet spheroid system

Jia Zhao, Shenghui Liang, Haoning Howard Cen, Yanjun Li, Robert K. Baker, …, Liangyi Chen, Yanmei Liu, Francis C. Lynn, James D. Johnson, Timothy J. Kieffer

Nature Communications  ·  13 Jul 2024  ·  doi:10.1038/s41467-024-50109-2

Remodeling ceramide homeostasis promotes functional maturation of human pluripotent stem cell-derived β cells

Huijuan Hua, Yaqi Wang, Xiaofeng Wang, Shusen Wang, Yunlu Zhou, …, Chao Tang, Zhongyang Shen, Cheng Li, Yuanyuan Du, Hongkui Deng

Cell Stem Cell  ·  01 May 2024  ·  doi:10.1016/j.stem.2024.04.010

Readily releasable β cells with tight Ca2+–exocytosis coupling dictate biphasic glucose-stimulated insulin secretion

Xiaohong Peng, Huixia Ren, Lu Yang, Shiyan Tong, Renjie Zhou, …, Tao Xu, Chao Tang, Zhixing Chen, Huisheng Liu, Liangyi Chen

Nature Metabolism  ·  26 Jan 2024  ·  doi:10.1038/s42255-023-00962-0

2023

Bright and sensitive red voltage indicators for imaging action potentials in brain slices and pancreatic islets

Yi Han, Junqi Yang, Yuan Li, Yu Chen, Huixia Ren, …, Beichen Xie, Mengying Deng, Yinghan Xiao, Jun Chu, Peng Zou

Science Advances  ·  24 Nov 2023  ·  doi:10.1126/sciadv.adi4208

A tool kit of highly selective and sensitive genetically encoded neuropeptide sensors

Huan Wang, Tongrui Qian, Yulin Zhao, Yizhou Zhuo, Chunling Wu, …, Chao Tang, Yingjie Zhu, Dayu Lin, Jiang-Ning Zhou, Yulong Li

Science  ·  17 Nov 2023  ·  doi:10.1126/science.abq8173

2022

Pancreatic α and β cells are globally phase-locked

Huixia Ren, Yanjun Li, Chengsheng Han, Yi Yu, Bowen Shi, …, Shufang Wu, Xiaojing Yang, Sneppen Kim, Liangyi Chen, Chao Tang

Nature Communications  ·  28 Jun 2022  ·  doi:10.1038/s41467-022-31373-6

A Multiclassifier System to Identify and Subtype Congenital Adrenal Hyperplasia Based on Circulating Steroid Hormones

Lei Ye, Zhiyun Zhao, Huixia Ren, Wencui Wang, Wenzhong Zhou, …, Zhihan Wan, Chao Tang, Shouyue Sun, Weiqing Wang, Guang Ning

The Journal of Clinical Endocrinology & Metabolism  ·  05 May 2022  ·  doi:10.1210/clinem/dgac271

Human pluripotent stem-cell-derived islets ameliorate diabetes in non-human primates

Yuanyuan Du, Zhen Liang, Shusen Wang, Dong Sun, Xiaofeng Wang, …, Cheng Li, Chao Tang, Zhongyang Shen, Xiaozhong Peng, Hongkui Deng

Nature Medicine  ·  01 Feb 2022  ·  doi:10.1038/s41591-021-01645-7

Cell-to-cell variability in inducible Caspase9-mediated cell death

Yuan Yuan, Huixia Ren, Yanjun Li, Shanshan Qin, Xiaojing Yang, Chao Tang

Cell Death & Disease  ·  10 Jan 2022  ·  doi:10.1038/s41419-021-04468-z

2021

Red‐ and Far‐Red‐Emitting Zinc Probes with Minimal Phototoxicity for Multiplexed Recording of Orchestrated Insulin Secretion

Junwei Zhang, Xiaohong Peng, Yunxiang Wu, Huixia Ren, Jingfu Sun, …, Yiwen Zhao, Shusen Wang, Chao Tang, Liangyi Chen, Zhixing Chen

Angewandte Chemie International Edition  ·  01 Dec 2021  ·  doi:https://doi.org/10.1002/anie.202109510

2020

Cellbow: a robust customizable cell segmentation program

Huixia Ren, Mengdi Zhao, Bo Liu, Ruixiao Yao, Qi Liu, Zhipeng Ren, Zirui Wu, Zongmao Gao, Xiaojing Yang, Chao Tang

Quantitative Biology  ·  01 Sep 2020  ·  doi:10.1007/s40484-020-0213-6

Pancreatic α and β cells are globally phase-locked

Huixia Ren, Yanjun Li, Chengsheng Han, Yi Yu, Bowen Shi, …, Shufang Wu, Xiaojing Yang, Sneppen Kim, Liangyi Chen, Chao Tang

Cold Spring Harbor Laboratory  ·  17 Aug 2020  ·  doi:10.1101/2020.08.16.252304

Cellbow: a robust customizable cell segmentation program

Huixia Ren, Mengdi Zhao, Bo Liu, Ruixiao Yao, Qi liu, Zhipeng Ren, Zirui Wu, Zongmao Gao, Xiaojing Yang, Chao Tang

Cold Spring Harbor Laboratory  ·  22 Apr 2020  ·  doi:10.1101/2020.04.21.052597

2014

Large-field high-resolution two-photon digital scanned light-sheet microscopy

Weijian Zong, Jia Zhao, Xuanyang Chen, Yuan Lin, Huixia Ren, …, Ming Fan, Zhuan Zhou, Heping Cheng, Yujie Sun, Liangyi Chen

Cell Research  ·  26 Sep 2014  ·  doi:10.1038/cr.2014.124